參觀者可以看到克里斯托弗-羅賓、小熊維尼和小豬的插圖，這是E.H. Shepard於1927年為A.A. Milne的《Now we are Six》創作的。
Winnie the Pooh and the mock turtle from Alice's Adventures in Wonderland are part of exhibition at Longleat House in Wiltshire until 8 January.
The work of seven artists will be on show with a range of illustrations dating from the 1860s to the 1960s.
"This small exhibition provides a rare opportunity to see an important part of the Longleat Collection that isn't very well known," curator James Ford said.
The exhibition, called Picture This: Illustrating Stories for Children, aims to celebrate children's books, and original drawings will be placed alongside the published books they appear in.
"Thanks to the 6th Marquess' passion for children's literature, we're lucky to have significant works by some of the most important artists ever to illustrate children's books," said Mr Ford.
"We hope guests enjoy seeing illustrations that brought to life children's classics such as Alice in Wonderland and Winnie the Pooh," he added.
A drawing of the mock turtle, created in 1866 by John Tenniel for Lewis Carroll's novel Alice's Adventures in Wonderland, is the earliest of the works on display.
Visitors can view an illustration of Christopher Robin, Winnie the Pooh and Piglet, created by E.H. Shepard in 1927 for A.A. Milne's Now we are Six.
Mr Crane's little-known Longleat black book of 56 watercolours, which he created for his six-year-old son Lionel in 1882, will be on display for the first time in the exhibition.